bend 3 immortalized mouse brain microvascular endothelial cell line  (ATCC)

Journal: Neurobiology of disease

Article Title: Multi-faceted therapeutic strategy for treatment of Alzheimer’s disease by concurrent administration of etodolac and α-tocopherol

doi: 10.1016/j.nbd.2019.01.020

Figure Lengend Snippet: Combination (COMB) treatment significantly increased tight junction proteins expression in vitro in bEnd3 cells and in vivo in isolated mice brain microvessels compared to vehicle treated groups (control = CTRL), and etodolac (ETO) and/or α-tocopherol (Tph).

Article Snippet: Cell culture Immortalized mouse brain endothelial cell line bEnd3 was obtained from ATCC (Manassas, VA). bEnd3 cells (passage 25–35) were cultured in DMEM supplemented with 10% FBS, and penicillin G (100 IU/ml)/streptomycin (100 g/ml).

Techniques: Expressing, In Vitro, In Vivo, Isolation, Control

Journal: Neurobiology of disease

Article Title: Multi-faceted therapeutic strategy for treatment of Alzheimer’s disease by concurrent administration of etodolac and α-tocopherol

doi: 10.1016/j.nbd.2019.01.020

Figure Lengend Snippet: Combination (COMB) treatment differentially and significantly altered Aβ transport across the cell-based BBB model associated with increased expression of Aβ major transport proteins in vitro in bEnd3 cells, and in vivo in isolated mice brain microvessels compared to control (CTRL), and etodolac (ETO) and/or α-tocopherol (Tph).

Article Snippet: Cell culture Immortalized mouse brain endothelial cell line bEnd3 was obtained from ATCC (Manassas, VA). bEnd3 cells (passage 25–35) were cultured in DMEM supplemented with 10% FBS, and penicillin G (100 IU/ml)/streptomycin (100 g/ml).

Techniques: Expressing, In Vitro, In Vivo, Isolation, Control